Cytokeratin 9

CK9 can be detected in primary cultures of palmoplantar keratinocytes when they shift to differentiation-promoting conditions and grow stratified (upper cells). CK9 has not been found in normal, i.e. non-pathogenic, non-ridged epidermis, beside some minor cells surrounding the acrosyringeal ducts. \n\nApplications:\nSuitable for use in Immunoblot (Western), Immunofluorescence and Immunohistochemistry (frozen tissue for paraffin-embedded sections, microwave treatment and in some cases protease digestion is recommended). The antibody cocktail is also an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn treatment.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Dilution: \nWestern Blot: 1:25-1:300 (chemiluminescence, AP/ECL).\nImmunofluorescence Microscopy: 1:10\nImmunohistochemistry: 1:20 (PAP/ABC).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-43A

Size

1ml

Applications

IF, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid.

P Type

Mab

Purity

Hybridoma culture supernatant

Isotype

IgG1+IgG3

References

1. Langbein, L., et al., Differentiation 55: 57-71 (1994). 2. Swensson, O., et al., Br. J. Dermatol. 139: 767-775 (1998). 3. Mochida, K., et al., Developmental Biology 227: 510-519 (2000).

Additional Info

Recognizes human Cytokeratin 9 (in mouse: some background staining). Antibody represents an excellent marker to study palmoplantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/ granulosum) of the epidermis of palm and sole. No labelling has been found in epithelial cells of other stratified epithelia such as esophagus or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. Negative tissues include: muscle, liver and duodenum.