Cytokeratin, Basal Cell (Basal Cell Cytokeratin)

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40kD-68kD and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. \n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10-1:25\nImmunohistochemistry (Frozen): 1:10-1:25\nImmunocytochemistry: 1:10-1:25\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-03C

Size

1ml

Applications

FC, ICC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can, Ch/Bird, GP, Prc, Rabbit

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Feitz, W. F., Debruyne, F. M., Vooijs, G. P., Herman, C. J., and Ramaekers, F. C. (1986). Intermediate filament proteins as tissue specific markers in normal and malignant urological tissues, J Urol 136, 922-31. \n2. Verhagen, A. P., Aalders, T. W., Ramaekers, F. C., Debruyne, F. M., and Schalken, J. A. (1988). Differential expression of keratins in the basal and luminal compartments of rat prostatic epithelium during degeneration and regeneration, Prostate 13, 25-38. 3. Schaafsma, H. E., Ramaekers, F. C., van Muijen, G. N., Ooms, E. C., and Ruiter, D. J. (1989). Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract, Histochemistry 91, 151-9. 4. Smedts, F., Ramaekers, F., Robben, H., Pruszczynski, M., van Muijen, G., Lane, B., Leigh, I., and Vooijs, P. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68. 5. Kuijpers, W., Tonnaer, E. L., Peters, T. A., and Ramaekers, F. C. (1991). Expression of intermediate filament proteins in the mature inner ear of the rat and guinea pig, Hear Res 52, 133-46. 6. Vos, J. H., van den Ingh, T. S., de Neijs, M., van Mil, F. N., Ivanyi, D., and Ramaekers, F. C. (1992). Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-) endocrine tissues, choroid plexus and spinal cord, J Vet Med 39, 721-40. 7. Vos, J. H., van den Ingh, T. S., Ramaekers, F. C., Molenbeek, R. F., de Neijs, M., van Mil, F. N., and Ivanyi, D. (1993). The expression of keratins, vimentin, neurofilament proteins, smooth muscle actin, neuron-specific enolase, and synaptophysin in tumors of the specific glands in the canine anal region, Vet Pathol 30, 352-61. 8. Verhagen, A. P., Ramaekers, F. C., Aalders, T. W., Schaafsma, H. E., Debruyne, F. M., and Schalken, J. A. (1992). Colocalization of basal and luminal cell-type cytokeratins in human prostate cancer, Cancer Res 52, 6182-7. 9. van Leenders, G., Dijkman, H., Hulsbergen-van de Kaa, C., Ruiter, D., and Schalken, J. (2000). Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy, Lab Invest 80, 1251-8.

Additional Info

Recognizes Basal Cell Cytokeratin. Stains basal cells in combined and stratified epithelial tissues. Species Crossreactivity: human, quail, chicken, rat, rabbit, hamster, canine, porcine, guinea pig