Cytokeratin, Basal Cell

Supplier:

United States Biological

Cat no: C9097-03B

Cytokeratin, Basal Cell

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in epithelial tissues by at least 20 different polypeptides. They range in molecular weight from between 40kD and 68kD and isoelectric pH between 4.9-7.8. The individual cytokeratin polypeptides are designated 1-20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100-1:1000\nImmunohistochemistry (frozen tissue sections): 1:100-1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol , aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

C9097-03B

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Can, Ch/Bird, Prc, Rabbit

Form

Supplied as a liquid in buffer with 0.09% sodium azide.

P Type

Mab

Purity

Purified by Protein G chromatography.

Isotype

IgG1

References

Smedts, F. et al. Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am. J. Path. (1990) 136:657

Additional Info

Broadly reacting cytokeratin antibody specific for cytokeratin 5. Stains basal cells in combined and stratified epithelial tissues. Recognizes the stem cell population, including the amplifying cell in the prostate epithelium. Species crossreactivity: human, rat, rabbit, hamster, canine, chicken, quail and porcine.

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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