Cytokeratins make up the largest family among the cytoplasmic intermediate filaments (IF) proteins and are expressed specifically in epithelial cells in a cell-specific manner. Cytokeratins include more than 20 unique gene products (termed K1-K20) that are divided into type I (K9-K20) and type II (K1-K8). This antibody recognizes cytokeratin polypeptides of 45 kD and 56 kD, corresponding to cytokeratin 18 and 10 respectively. Cytokeratin 18 is a type-I keratin that is found in a variety of simple epithelial tissues. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. It maps to 12q13 region of human chromosome. It is expressed in colon, placenta, liver and very weakly in exocervix. Cytokeratin 10 is also a member of the type I (acidic) cytokeratin family. It is synthesized in the suprabasal cell layers of certain stratified epithelia, in an endogenous differentiation program and is expressed in certain epithelial tumors and is generally associated with keratin-1. Its deficiency leads to epidermolytic hyperkeratosis (EHK).
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution::
Immunohistochemistry (formalin fixed paraffin embedded): 1:25-1:50
Optimal dilutions to be determined by the researcher.
Positive Control:
Skin
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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