Cytokeratin, Epithelial

Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative MW of 40-68kD (1,2). These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central a-helical rod that is flanked by N- and C-terminal domains at each end (3). Generally, low MW keratins are expressed in the early stages of cell development, whereas high MW keratins are expressed in maturing cells (4,5). Anti-cytokeratin has been used to characterize epithelial origin of adenomatoid tumors (8), to distinguish undifferentiated carcinomas from large cell lymphomas (9), and to discern between undifferentiated carcinoma and malignant melanoma when combined with S-100 antibody\n(10). Positive staining of keratin proteins in embryonal carcinomas, endodermal sinus tumors, and choriocarcinomas distinguishes these carcinomas from seminomas.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (Frozen and formalin-fixed paraffin-embedded tissue sections): Expect staining mainly in the cytoplasm. Note: Epitope Retrieval required using EDTA, pH 8 or enzyme digestion. \nOptimal dilutions to be determined by the researcher.\n\nSource: Ascites\n\nPositive Control Tissue: Normal Human Skin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nRelated Products:\nC9097-47: Cytokeratin, Epithelial, pan Mab Mo xHu\nC9097-55: Cytokeratin, Epithelial Mab Mo xHu

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SPECIFICATIONS

Catalog Number

C9097-56

Size

1ml

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide, before the addition of 40% glycerol.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Moll, R., et al., Cell 31:11-24, 1982. 2. Moll, R., et al., Am J Pathol. 140(2): 427-47, 1992. 3. Steiner, P.M., et al., Cell 42:411-9 (1985). 4. Sunn, T.T., et al., J. Invest. Dermatol. 81:109s-115s, 1983. 5. Clausen, H., et al., J. Invest. Dermatol. 86(3): 249-254, 1986. 6. Tseng, S.C.G., et al., Cell 30:361-372, 1982. 7. McCormick, S.R., et al., Am. J. Clin.P athol. 110:227-237, 1998. 8. Wu, Y., et al., Cell 31:693-703, 1982. 9. Battifora, H., et al., Hum. Pathol. 11:635-41, 1980. 10. Ramaekers, F.C.S., et al., J. Clin. Invest. 71:635-42, 1983. 11. Krill, D., et al., Urology 49(6): 981-988, 1997. 12. O

Additional Info

Reacts with keratin proteins in the basic subfamily (65-67, 64, 59, 58, 56 and 52kD). Stains the whole epithelium of most tissue such as skin, esophagus, and bladder. Also stains the ducts and parenchymal cells of pancreas. Does not stain of mesenchymal cells (6).