Cytokeratin, Epithelial, pan

Cytokeratin filaments consist of a complex family of approximately 20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells. This antibody may be useful in determining if a neoplasm originated from an epithelial or non-epithelial source, and is especially useful for the distinction of metastatic malignant tumors of epithelial origin.\n\nApplications: \nSuitable for use in Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: 10-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nAdditional Specificity:\nThe antibody reacts with epithelial and mesothelial cells. Exception: Secretory cells are not stained and acinus cells, for example, show only weak staining. The antibody does not react with connective tissue, blood or lymphatic vessels, smooth or skeletal muscles. Exception: When higher antibody concentrations are used, weakly positive reactions can be observed with the smooth muscles of the myometrium, the prostata as well as the gastric muscularis mucosa. The antibodies can be used for differential diagnosis of epithelial tumors, distinction from mesenchymal, neural tumors as well as large-cell lymphomas. Anti-cytokeratin pan stains all epithelial tumors (primary tumors as well as metastases). Exception: Only partially positive staining is observed with theca cell tumors, granulosa cell tumors, adrenal cortex tumors. In mixed tumors (e.g. carcinosarkoma, thymoma, adenolymphoma) only the epithelial regions are stained. Tumors of nonepithelial origin do not react with the antibody. Exception: With high antibody concentrations a weak staining of leiomyosarcoma of the uterus is observed.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C9097-46

Size

100ug

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.1% sodium azide, before the addition of 40% glycerol.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Overbeck, J., et al. (1985) Virchows Arch. Abt. A. Pathol. Anat. 407: 1-12.

Additional Info

Reacts with an epitope which is common to all cytokeratins respectively with a cytokeratin-associated protein. The antigen recognized by anti-cytokeratin pan is present in all types of epithelia. See additional Specificity information.