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Cytokeratin, Pan

Cat no: C9097-01S

Cytokeratin, Pan

Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells. \n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Frozen, paraffin): 1:75-1:200 for paraffin. Incubate 30-60 minutes at RT. Requires Proteolytic enzymatic pretreatment. \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C9097-01S

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum, Bov

Form

Supplied as a liquid in PBS, pH 7.5, 15mM sodium azide.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

Recognizes bovine cytokeratins of MW 58, 56, 52, 60, 51, 48 and 68kD. Recognizes a broad spectrum of human keratins. Species Crossreactivity: human.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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