Cytokeratin filaments consist of a complex family of ~20 different cytokeratin polypeptides with relative molecular weights of 40kD to 68kD. These intracellular intermediate filaments of 10nm in diameter provide structural integrity to epithelial cells. All the filament subunits share a general common fibrillar organization, consisting of a central alpha-helical rod that is flanked by N- and C-terminal domains at each end. Generally, low molecular weight keratins are expressed in the early stages of cell development, whereas high molecular weight keratins are expressed in maturing cells.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): 1:75-1:200 for paraffin. Incubate 30-60 minutes at RT. Requires Proteolytic enzymatic pretreatment.
Optimal dilutions to be determined by the researcher.
Positive Control:
Skin
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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