Cytosine deaminase belongs to the cytidine and deoxycytidylate deaminase family. This enzyme converts cytosine to uracil or 5-methylcytosine to thymine by deaminating carbon number 4. It is present in prokaryotes and fungi but not in multicellular eukaryotes and is an important member of the pyrimidine salvage pathway. The enzyme is of widespread interest both for antimicrobial drug design and for gene therapy applications against tumors.
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blot: 1:2000
Immunohistochemistry (Frozen): 10ug/ml; Sample DNA must be denatured before using C9110-01A. Fixation in cold methanol for 30 minutes followed by immersion in 7x10e3N Sodium hydroxide for 10-15 seconds allows staining with the simultaneous detection of nuclear, cytoplasmic and membrane assigns as well as preservation of morphological detail.
Immunoprecipitation: 25-100ug/ml Tested using C910-01A, bromodeoxyuridine (BrdU) or a control (no antigen)
Chromatin Immunoprecipitation: 5ul for 10e6 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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