D4-GDI, Recombinant, Human (D4 GDP-Dissociation Inhibitor, RhoGDI)

D4-GDI belongs to the family of rho-GDI or GDP-dissociation inhibitors for the Rho/Rac family of small G proteins; consisting of rhoGDI-1, D4-GDI/rhoGDI-2 and rhoGDI-3. It inhibits the release of GDP from the Rho proteins, essential for GTP-loading and activation of GTPase activity. D4-GDI is located in the cytoplasm and in involved in the regulation of the membrane association and dissociation cycle of Rho/Rac proteins. It is known to be a substrate for caspase 3 during Fas-mediated apoptosis.\n\nRecombinant rho-GDI shows a band at 54kD on SDS-PAGE commassie blue stained gel.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by coomassie blue SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of Human D4-GDI.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D0100-07

Size

2ug

Form

Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

(same/more than) 90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. The p75 receptor acts as a displacement factor that releases Rho from Rho-GDI. Yamashita T, Tohyama M, Nat Neurosci 2003 May;6(5):461-7 2. Regulation of GRIP1 and CBP Coactivator activity by Rho GDI modulates estrogen receptor transcriptional enhancement. Su LF, Wang Z, J Biol Chem 2002 Oct 4;277(40):37037-44 3. Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI. Del Pozo MA, Kiosses WB, Meller N, Nat Cell Biol 2002 Mar;4(3):232-9 4. Interactions between Rho GTPases and Rho GDP dissociation inhibitor (Rho-GDI). Faure J, Dagher MC, Biochimie 2001 May;83(5):409-14 5. Maintenance of CDC42 GDP-bound state by Rho-GDI inhibits MAP kinase activation by the exchange factor Ras-GRF. evidence for Ras-GRF function being inhibited by Cdc42-GDP but unaffected by CDC42-GTP. Arozarena I, Matallanas D, J Biol Chem 2001 Jun 15;276(24):21878-84 6. Cloning of Rac and Rho-GDI from tobacco using an heterologous two-hybrid screen. Kieffer F, Elmayan T, Simon-Plas F, Biochimie 2000 Dec;82(12):1099-105.