Dab1, phosphorylated (Tyr198) (Disabled homolog 1 Drosophila, Scm, Scr, Scrambler, Yot, Yotari)

Disabled 1 (Dab1) is an 80kD protein that is encoded by the Disabled 1 gene locus which is mutated in scrambler and yotari mutant mice. Phenotypically, the mutation of this gene produces motor defects and ataxia, disruption of neuronal migration, and severe cerebellar hypoplasia. Dab1 is an intracellular adapter protein that functions in downstream signaling events initiated by the secreted protein reelin. Dab1 contains a phosphotyrosine binding (PTB) domain in the amino terminus. Tyrosine phosphorylation of Dab1 is increased by reelin binding to the Very Low Density Lipoprotein Receptor (VLDLR) and Apolipoprotein E Receptor 2 (ApoER2) through stimulation of Src family kinases (e.g., Fyn). Src family kinase and c-Abl activities are themselves then stimulated by binding to tyrosine phosphorylated Dab1. Dab1 also mediates activation of Akt (PKB) by reelin resulting in inhibition of glycogen synthase kinase 3 beta (GSK-3 beta) and decreased phosphorylation of the microtubule-associated protein, Tau. Dab1 tyrosine 198 is a major site for reelin-induced Src family kinase-mediated phosphorylation in embryonic neurons.\n\nPositive Control: Human Embryonic Kidney (HEK293) cells transfected with wild-type mouse Dab1 protein.\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D0900-14C

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Ch/Bird

Form

0.05% Sodium Azide; 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

References

Arnaud L et al. Fyn tyrosine kinase is a critical regulator of disabled-1 during brain development. Curr Biol 13:9-17 (2003). <PUBMED:12526739>Beffert U et al. Reelin-mediated signaling locally regulates protein kinase B/Akt and glycogen synthase kinase 3beta. J Biol Chem 277:49958-64 (2002). <PUBMED:12376533>Keshvara L et al. Identification of reelin-induced sites of tyrosyl phosphorylation on disabled 1. J Biol Chem 276:16008-14 (2001). <PUBMED:11279201>Howell BW et al. Dab1 tyrosine phosphorylation sites relay positional signals during mouse brain development. Curr Biol 10:877-85 (2000). <PUBMED:10959835>\n5. Hiesberger, T., et al. Direct binding of reelin to VLDL receptor and ApoE receptor 2 induces tyrosine phophorylation od disabled-1 and modulates tau phosphorylation. Nueron. 24(2): 481-489, 1999.\n6. Homayouni, R., et al. Disabled-1 binds to the cytoplasmic domain of amylodi precursor-like protein 1. J. Neurosci. 19(17): 7507-7515, 1999.\n7. Howell, B.W., et al. Reelin-induced tyrosine phosphorylation of disabled 1 during neuronal positioning. Genes Dev. 13(6): 643-648, 1999.

Additional Info

Recognizes Dab1 when phosphorylated at Tyr198. Species Crossreactivity: Crossreacts with Mouse. Expected to crossreact with Chicken (100% identity with immunogen), Rat (100% identity with immunogen), and human (100% identity with immunogen) due to sequence homology.

Alternative Names

Disabled homolog 1 (Drosophila), Scm, Scr, Scrambler, Yot, Yotari