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DACT1 (Dapper Homolog 1, hDPR1, Hepatocellular Carcinoma Novel Gene 3 Protein, DPR1, HNG3)

Cat no: D1055-02A

DACT1 (Dapper Homolog 1, hDPR1, Hepatocellular Carcinoma Novel Gene 3 Protein, DPR1, HNG3)

Dapper homolog 1, an ortholog of Xenopus Dpr, antagonizes Wnt signaling by promoting Dvl degradation via a lysosome inhibitor-sensitive and proteasome inhibitor-insensitive mechanism. Dapper homolog 1 acts as an interacting protein for Dvl, and the interaction is formed between the DEP (Dishevelled, Egl-10, and pleckstrin) domain of Dvl and the central and C-terminal regions of Dapper homolog 1. The gene for human Dapper homolog 1 is localized on chromosomal region 14q23.1. The protein impedes the degradation of CTNNB1/beta-catenin, thereby enhancing the transcriptional activation of target genes of the Wnt signaling pathway. The protein is also required for normal notochord formation and is downregulated in hepatocellular carcinomas (HCC). It is expressed in human liver and various other tissues.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-1ug/ml\nOptimal dilutions to be determined by the researcher. \n\nPositive Control:\nHuman, mouse and rat brain lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1055-02A

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird, Xen/Amph, ZF/Fish

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Zhang, L. et al. J. Biol. Chem. 281:8607-8612 (2006). 2. Cheyette, BN. et al. Dev. Cell. 2:449-461 (2002).

Additional Info

Recognizes human Dapper homolog 1. Species Crossreactivity: chicken, chimpanzee, mouse, rat, xenopus, zebrafish.

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Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info
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