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DACT3 (Dapper Homolog 3, MGC15476, RRR1, Thymus Expressed Gene 3-like)

Cat no: D1055-01B


Supplier: United States Biological
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DACT3, a member of the DACT (Dpr/Frodo) gene family, plays an important role in a negative regulator of Wnt/-catenin signaling. Unlike other Wnt signaling inhibitors that are silenced by DNA methylation, DACT3 repression is associated with bivalent histone modifications. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:125 Western Blot: 1:125-1:250 Optimal dilutions to be determined by the researcher. Hybridoma: F0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: D1055-01B
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Western Blot
Size: 50ul
Form: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
P type: Mab
Isotype: IgG1,k
Purity: Purified by immunoaffinity chromatography.
References: 1. Jiang X, et al., (2008) Cancer Cell. 13(6):529
Additional info: Recognizes human DACT3 (89-202aa) at ~64kD.

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