DAP Kinase 2, NT (DAK2, DAP Kinase Related Protein 1, DAPK2, Death Associated Protein Kinase 2)

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependent on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2000\nImmunohistochemistry: 1:50-100\nFlow Cytometry: 1:15\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

D1052-01E

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-glycine, pH 7.4, 0.15M sodium chloride, 40% glycerol, 0.01% sodium azide, 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Kawai T, et al. Oncogene 18(23):3471-80, 1999. 2. Inbal B, et al. Mol Cell Biol 20(3):1044-54, 2000.\n3. Shani G, et al. EMBO J 20(5):1099-113, 2001.

Additional Info

Recognizes human DAPK2.