DAP3 (Death Associated Protein 3)

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Splice variants that differ in the 5' UTR have been found for this gene; both variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:16,000\nWestern Blot: 1-3ug/ml ~40kD band observed in lysates of cell line HeLa (calculated MW of 45.6kD according to NP_387506.1). In transfected HEK293 transiently expressing DAP3 a band of approx. 48kD is observed. This band is not observed in the non-transfected HEK293.\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1049-03F

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1.Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G, Kimchi A, Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death.J Biol Chem. 1995 Nov 17;270(46):27932-6.

Additional Info

Recognizes human Death Associated Protein 3.

Alternative Names

mitochondrial 28S ribosomal protein S29, death-associated protein 3, bMRP-10, MRPS29, MRP-S29, MGC126059, MGC126058, DKFZp686G12159, DAP-3, death associated protein 3, DAP3