DAP3 (Death Associated Protein3)

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively (2-3). Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction (3). \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nWhole cell lysate from Jurkat cells can be used as positive control and an approximatley 45kD band should be detected. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1049-03B

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG

References

1. Levy-Strumpf N, Kimchi A. Oncogene 24: 3331-3340 (1998). 2. Kimchi A. Ann Rheum Dis 58 suppll 1: I14-19 (1999). 3. Kissil JL, Deiss LP, Bayewitch M, Raveh T, Khaspekov G and Kimchi A. J. Biol. Chem. 270: 27932-27936 (1995). 4. Berger T, Brigl M, Herrmann JM, Vielhauer V, Luckow B, Schlondorff D, Kretzler M. J Cell Sci 113 ( Pt 20):3603- 3612 (2000). 5. Miyazaki, T., Kim, Hyung-Ryong, Godzik, A., Krajewski, S.,Reed, J. Nature Immunology 3, 4-5 (2002).

Additional Info

Recognizes mouse DAP3 (Death Associated Protein3)