DAP5 (Death Associated Protein 5, EIF4g2, Eukaryotic Elongation Factor 4 gamma 2)

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase, which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5) (1-2). DAP-5 (also named p97 and NAT1) is a ubiquitously expressed member of the translation initiation factor eIF4G family that lacks the eIF4E binding site. DAP-5 was cloned from HeLa cells using a functional approach in attempt to isolate cDNA fragments, which convey resistance to gamma interferon (IFN-g)-induced programmed cell death (3). A fragment of the cDNA when expressed as a mini-protein of 28kD protects the cell from IFN-g induced apoptosis. The full-length DAP-5 cDNA codes for a protein of approximate molecular weight of 97kD. Low level of expression protects the cells, whereas high level of expression results in cell death (3-4).\n\nPositive Control: Jurkat\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D1049-05B

Size

50ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

PBS containing 0.2% gelatin, 0.05% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Wittke I, Madge B, Wiedemeyer R, Kimchi A, Schwab M. Cancer Lett 162(2):237-243 (2001).\n2. Levy-Strumpf N, Kimchi A. Oncogene 24: 3331-3340 (1998).\n3. Kimchi A. Ann Rheum Dis 58 suppll 1: I14-19 (1999).\n4. Levy-Strumpf N, Deiss LP, Berissi H, Kimchi A. Mol Cell Biol 17(3):1615-1625 (1997).\n5. Berger T, Brigl M, Herrmann JM, Vielhauer V, Luckow B, Schlondorff D, Kretzler M. J Cell Sci 113 ( Pt 20):3603-3612 (2000).

Additional Info

In Jurkat, a 97kD band is observed. Species Crossreactivity: Crossreacts with Human and Mouse. Predicted to crossreact with Rat, Bovine, Canine and Monkey proteins based on 100% identity of the immunogen.