DAPK2, a DAPK type protein kinase, contains a N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to other kinases in this subfamily. In vitro assay experiments have shown that the protein is autophosphorylated. Overexpression of the DAPK2 gene was shown to induce cell apoptosis.
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.