DARC (CT) (Duffy Antigen/Chemokine Receptor, Duffy Antigen/Chemokine Receptor, Fy Glycoprotein D, GpFy, Cell Adhesion Molecule 3, CADM3, Plasmodium Vivax Receptor,CCBP1, CD234, CD234 antigen, Dfy, GpFy, Plasmodium vivax receptor)

CD234 is a non-specific receptor for a number of cytokines including IL-8, GRO, RANTES, MCP-1 and TARC. CD234 also functions as the receptor for the malaria parasites Plasmodium vivax and P. knowlesi. CD234 is also responsible for the Duffy blood group system where a substitution at position 42 of either Gly-42 or Asp-42 results in Fy(A) or Fy(B). CD234 is present in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along post capillary venules throughout the body, except in the adult liver. Erythroid cells and post capillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. It has been demonstrated that CD234 exists in multiple isoforms.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nImmunohistochemistry (Paraffin): 2.5ug/ml Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer pH 6.0 is recommended. \nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies\nSheep Anti Rabbit IgG HRP\n\nImmunohistochemistry Positive Control Tissue:\nMouse brain\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1065-50H

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gardner, L., et al. (2004) The human Duffy antigen binds selected inflammatory but not homeostatic chemokines. Biochem. Biophys. Res. Commun. 321:306-12. 2. Chaudhuri, A., et al. (1993) Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite. PNAS 90:10793-7. 3. Gil, M.L., et al (1992) A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and functional studies. J. Immunol. 2826-33. J. Immunol. 148(9):2826-33

Additional Info

Recognizes CD234 in mouse brain tissue lysate at ~ 23 and 36kD. Species crossreactivity: mouse, rat