DAXX (BING2, DAP6, Daxx, Death domain-associated protein 6, EAP1, ETS1-associated protein 1, Fas death domain-associated protein)

DAXX (death-domain associated protein), it is a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants.\n\nApplications:\nWestern Bloting: 1:500-1:2000.\nImmunofluorescence: 1:200-1:1000.\nFlow cytometry: 1:200-1:400.\nELISA: Suggested dilution 1:10000.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

030139

Size

100ul

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.03% sodium azide, 50% glycerol.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Cell. 1997 Jun 27;89(7):1067-76.\n2. Biochem Biophys Res Commun. 2000 Dec 9;279(1):6-10. \n3. Proc Natl Acad Sci U S A. 2004 Aug 17;101(33):12130-5.

Additional Info

Human

Alternative Names

DAP6; EAP1; BING2