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DBC1 (Protein KIAA1967, Deleted in Breast Cancer Gene 1 Protein, DBC-1, DBC.1, p30 DBC, KIAA1967)

DBC1 (Protein KIAA1967, Deleted in Breast Cancer Gene 1 Protein, DBC-1, DBC.1, p30 DBC, KIAA1967)

Cat no: D1171-01F

Supplier: United States Biological
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Deleted in breast cancer gene 1 protein (DBC1) was originally identified by its localization to a region of chromosome 8p21 that is homozygously deleted in breast cancer (1). DBC1 is a large, nuclear protein with multiple functions in cell survival. It binds directly to the estrogen receptor a (ERa) hormone-binding domain in a ligand-independent manner and may be a key determinant of ligand-independent ERa expression and survival in human breast cancer cells (2). DBC1 can promote p53-mediated apoptosis by binding to and inhibiting the deacetylase activity of SirT1, resulting in increased p53 acetylation levels and activity (3). DBC1 may be an important regulator of heterochromatin formation as it binds SUV39H1 and inhibits its histone methyltransferase activity (4). Caspase-dependent processing activates the pro-apoptotic activity of DBC1 during Tumor Necrosis Factor-a (TNF-a)-mediated cell death signaling (5). This processing of DBC1 in response to TNF-a is an early event in the onset of apoptosis and results in relocalization of DBC1 to the cytoplasm. Over-expression of the processed, cytoplasmic form of DBC1 results in mitochondrial clustering and matrix condensation and sensitizes cells to TNF-a-mediated apoptosis. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: D1171-01F
Reactivities: Human, Mouse, Rat, Non-Human Primate
Hosts: Rabbit
Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.
P type: Pab
Purity: Purified by Protein A affinity chromatography.
References: 1.Hamaguchi, M. et al. (2002) Proc Natl Acad Sci USA 99, 13647-52. 2.Trauernicht, A.M. et al. (2007) Mol Endocrinol 21, 1526-36. 3.Zhao, W. et al. (2008) Nature 451, a587-90. 4.Li, Z. et al. (2009) J Biol Chem 284, 10361-6. 5.Sundararajan, R. et al. (2005) Oncogene 24, 4908-20.
Additional info: Recognizes endogenous levels of total human DBC1. Species Crossreactivity: Equine, monkey, mouse and rat.

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