DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85kD member of the Cys-Cys:His-His subfamily of zinc-finger proteins. This subfamily is principally involved in protein-DNA interactions. DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells. Human DC-SCRIPT is 744 amino acids (aa) in length. It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255-556) and an extended acidic region (aa 586-698). Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.
Applications:
Suitable for use in Flow Cytometry, Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 10ug/ml use 10ul to label 10e6 cells.
Direct ELISA: 0.5-1.0ug/ml detection limit for rhDC-SCRIPT is ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhDC-SCRIPT is ~ 5ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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