DC-SIGN, CT (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1)

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC- SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC- \nSIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. \n \nSOURCE: \nMouse monoclonal DC-SIGN antibody was raised against a \npeptide corresponding to amino acids near the carboxy \nterminus of human DC-SIGN (1). DC-SIGN antibody was \npurified from ascites from hybridoma clone 5D7. \n \nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 5-10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nJurkat cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

D1550-03

Size

100ug

Applications

IHC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Geijtenbeek TB, et al., Cell 2000; 100:587-97. 2. Pohlmann S, et al., J. Virol. 2001;75:4664-72. \n3. Geijtenbeek TB, et al.,. Cell 2000; 100:575-85. 4. Soilleux EJ, et al., J. Immunol. 2000 165:2937-42. \n5. Pohlmann S, et al., Proc Natl Acad Sci USA. 2001; 98:2670-2675. 6. Bashirova AA, et al., J. Exp. Med. 2001; 193:671-8. 7. Mitchell DA, et al., J Biol Chem. 2001; 276:28939-45. 8. Mummidi S, et al., J Biol Chem. 2001; 276:33196-212.

Additional Info

Recognizes human DC-SIGN.