DC-SIGN, ED (Dendritic Cells, CD209, Dendritic Cell-Specific ICAM-3, SIGNR1, SIGN-R1)

Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes. DCs also amplify the amount of virus and extend the duration of viral infectivity. Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC- SIGN (2). ICAM-3 is the natural ligand for DC-SIGN (3). A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC- SIGN2) was identified recently (4-8). DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8). The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum. \n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml, Detects a band of ~44kD.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-40J: IgG, Fc (HRP) Pab Gt xRb\nI1904-40P: IgG, Fc, X-Adsorbed (HRP) Pab Gt xRb\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

D1550-01

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Geijtenbeek TB, Kwon DS, Torensma R, et al. DC-SIGN, a dendritic cell-specific HIV-1-binding protein that enhances trans infection of T cells. Cell 2000; 100:587-97. \n2. Pohlmann S, Baribaud F, Lee B, et al. DC-SIGN interactions with human immunodeficiency virus type 1 and 2 and simian immunodeficiency virus. J. Virol. 2001;75:4664-72. \n3. Geijtenbeek TB, Torensma R, van Vliet SJ, et al. Identification of DC-SIGN, a novel dendritic cell-specific ICAM-3 receptor that supports primary immune responses. Cell 2000; 100:575-85. \n4. Soilleux EJ, Barten R, Trowsdale J. DC-SIGN; a related gene DC-SIGNR; and CD23 form a cluster on 19p13. J. Immunol. 2000 165:2937-42. \n5. Pohlmann S, Soilleux EJ, Baribaud F, et al. DC-SIGNR, a DC SIGN homologue expressed in endothelial cells, binds to human and simian immunodeficiency viruses and activates infection in trans. Proc Natl Acad Sci USA. 2001; 98:2670-2675. \n6. Bashirova AA, Geijtenbeek TB, van Duijnhoven GC, at el. A dendritic cell-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN)-related protein is highly expressed on human liver sinusoidal endothelial cells and promotes HIV-1 infection. J. Exp. Med. 2001; 193:671-8. \n7. Mitchell DA, Fadden AJ, Drickamer K. A novel mechanism of carbohydrate recognition by the C-type lectins DC-SIGN and DC SIGNR: Subunit organisation and binding to multivalent ligands. J Biol Chem. 2001; 276:28939-45. \n8. Mummidi S, Catano G, Lam L, et al. Extensive repertoire of membrane-bound and soluble DC-SIGN1 and DC-SIGN2 isoforms Inter-individual variation in expression of DC-SIGN transcripts. J Biol Chem. 2001; 276:33196-212.

Additional Info

Recognizes an epitope within the extracellular domain of human DC-SIGN. DC-SIGN was designated CD209 at the 7th leucocyte typing workshop.