DCAF6 (DDB1- and CUL4-associated Factor 6, Androgen Receptor Complex-associated Protein, ARCAP, IQ Motif and WD Repeat-containing Protein 1, Nuclear Receptor Interaction Protein, NRIP, IQWD1, MSTP055)

IQ motif and WD repeats 1 (IQWD1) WD-repeat proteins are a large family of eukaryotic proteins coordinating multi-protein complex assemblies and their role has been implicated in multiple cellular processes including signal transduction, transcriptional regulation, cell cycle control and apoptosis. NRIP is a novel 860aa nuclear protein consisting of seven conserved WD40 domains and one NLS motif. It binds to androgen and glucocorticoid receptors and up regulates their transcriptional activity, thereby functioning as a nuclear receptor coactivator. Role of NRIP has been implicated in cell growth and also in cervical and prostrate cancer thus indicating a therapeutic intervention. Northern Blot analysis detected a high expression of NRIP in skeletal muscle and testis and low expression in heart, prostrate and adrenal gland.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMuscle\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D1575-30

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Tsai,T.C., Lee,Y.L., Hsiao,W.C., Tsao,Y.P. and Chen,S.L. NRIP, a novel nuclear receptor interaction protein, enhances the transcriptional activity of nuclear receptors. J. Biol. Chem. 280 (20), 20000-20009 (2005). 2. Tureci,O., Sahin,U., Koslowski,M., Buss,B., Bell,C., Ballweber,P., Zwick,C., Eberle,T., Zuber,M., Villena-Heinsen,C., Seitz,G. and Pfreundschuh,M. A novel tumour associated leucine zipper protein targeting to sites of gene transcription and splicing. Oncogene 21 (24), 3879-3888 (2002).

Additional Info

Recognizes human NRIP. Species Crossreactivity: canine, mouse, rat. Species sequence homology: zebrafish, xenopus.