Dectin-1, also known as beta-glucan receptor (bGR), is a C-type lectin domain family member. It is a major pathogen pattern-recognition receptor that binds beta-glucans, glucose polymers that form cell-wall components or exopolymers of yeasts, fungi and some bacteria. Beta-glucans have also been used experimentally and therapeutically as immunomodulators that enhance resistance to bacterial, yeast, viral and protozoan infections, as well as tumor formation. Dectin-1 also acts as a co-stimulatory molecule on T-cells to induce their proliferation and is necessary for the TLR2-mediated inflammatory response. It is highly expressed on peripheral blood leukocytes and dendritic cells.
Human Dectin-1 has two major (bGR-A and bGR-B) and several minor isoforms. bGR-A and bGR-B differs by the presence and absence, respectively, of a stalk region. D1876-48G recognizes bGR-A only.
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:25-1:200; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.
Hybridoma:
Spleen cells from immunized C57BL/6 were fused with cells of the murine SP2 myeloma cell line.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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