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Products Demethylase (Jumonji-type) Activity Assay Kit
| Catalogue number: | 700390 |
| Weight: | 0 |
| Form: | 96 Well |
| P type: | Assay Kits|Demethylase |
| Shipping temp: | -80 |
| Storage temp: | -80 |
| Additional info: | Lysine demethylases containing Jumonji C (JmjC) domains produce formaldehyde following 2-oxoglutarate-dependent demethylation. Cayman's Demethylase (Jumonji-type) Activity Assay provides a convenient fluorescence-based method for assaying JmjC-mediated demethylase activity from cell lysates or purified enzyme preparations. The assay is based on the production of formaldehyde during the demethylation of a methylated peptide substrate. Cyclization of formaldehyde and acetoacetanilide in the presence of ammonia gives a fluorescent product for quantitation. |
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