DFF45 (NT) (ICAD, DNA Fragmentation Factor, Inhibitor of Caspase Activated Deoxyribonuclease)

Apoptosis, the process of programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms and involves dramatic changes in cellular structure. Disruption of apoptosis is also related to many diseases. It is induced by a family of cell death receptors and their ligands. Cell death signals are then transduced adapter molecules and members of the caspase (ICE/CED-3) family of cysteine proteases that eventually cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF45) was identified recently (2). Its mouse homologue was designated ICAD (1,3). DFF45 and ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (2). DFF is composed of two subunits; DFF40/CAD and DFF45/ICAD. Cleavage of DFF45/ICAD which is mediated by caspase-3 leads to DFF40/CAD activation to function as a nuclease (1

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SPECIFICATIONS

Catalog Number

D3224-06

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.2, containing 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Enari, M., Sakahira, H., Yokoyama, H., Okawa, K., Iwamatsu, A. and Nagata, S. (1998) \nNature 391: 43-50. \n2. Liu, X., Zou, H., Slaughter, C. and Wang, X. (1997) Cell 89: 175-184. \n3. Sakahira, H., Enari, M. and Nagata, S. (1998) Nature 391: 96-99. \n4. Wyllie, A. (1998) Nature 391: 20-21. \n

Additional Info

This immunoaffinity purified antibody Detects a ~47kD protein, corresponding to the apparent molecular mass of ICADL and a 28kD protein, corresponding to the apparent molecular mass of ICADS, on SDS-PAGE immunoblots, in mouse and rat tissue \nextracts. Additional bands at approximately 60kD and 40kD, are also observed on immunoblots.