DGK-Z (Diacylglycerol kinase zeta; also DAG kinase 6) is a 130kD member of the eukaryotic diacylglycerol (DAG) kinase family of proteins. It exists in both cytoplasm and nucleus, and is widely expressed, being found in neurons, skeletal muscle cells and endothelium. DGK-Z converts DAG into phosphatidic acid (PA). In the nucleus, this activates mTOR and promotes cell proliferation. In the cytoplasm, DGK-Z-generated PA activates PAK1, leading to increased cell motility. Human DGK-Z is 1117aa in length and contains two DAG-type Zn-finger regions (aa287-419), a MARCKS homology domain (aa448-462) and a catalytic domain (aa486-607). In addition to the 130kD protein, there are several splice variants that run about 114kD in SDS-PAGE.
Applications:
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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