DHEAS (Dehydroepiandrosterone Sulphate)

Cat no: D3228-03

Supplier: United States Biological

0 reviews | Write a Review Pencil

Read More on Supplier Website

Description
Additional Information

Dehydroepiandrosterone is the most abundant adrenal androgen in humans and exists predominately in a sulfated form (DHEAS) [1]. DHEA sulfotransferase, known as SULT2A1, converts the androgen precursor DHEA to its inactive sulfate ester, DHEAS. The unconjugated molecules can be converted directly to androgens. Dehydroepiandrosterone is converted to DHEAS in the adrenal glands and liver [2]. DHEA in adults is present at plasma concentrations 100 to 500 times higher than those of testosterone. DHEA can be metabolized to androgens and/or estrogens in the prostate [3]. Hepatic metabolites include 16 alpha-hydroxy-DHEA, 7 alpha-hydroxy-DHEA, and 7-oxo-DHEA [4]. 7 beta-OH-DHEA is a metabolite [5]. The major metabolite produced in humans is a mono-hydroxylated DHEA species [4]. Synthesis of a 7 alpha-hydroxy-DHEA and 5-androstene-3 beta, 17 beta-diol occurs in the frontal cortex, hippocampus, amygdala, cerebellum and striatum [6]. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1.25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Catalogue number:	D3228-03
Hosts:	Sheep
Applications:	ELISA
Size:	100ul
Form:	Supplied as a liquid in 20mM phosphate, 150mM sodium chloride, pH 7.2, 0.09% sodium azide.
P type:	Pab
Isotype:	IgG
Purity:	Purified
References:	1. Weiss EP, Shah K, Fontana L, Lambert CP, Holloszy JO, Villareal DT (2009) Dehydroepiandrosterone replacement therapy in older adults; 1-2y effects on bone. Am J Clin Nutr. 89(5): 1459-67 (0798/lr/0001). 2. Noordam C, Dhir V, McNelis JC , Schlereth F, Hanley NA, Krone N, Smetitink JA, Smeets R, Sweep FCGJ, Claahsen-van der Griten HL, Arlt W (2009) Inactivating PAPSS2 metations in a patients with premature pubarche. The New England Journal of Medicine. 360:2310-8 (0798/lr/0002). 3. Gray NE, Liu X, Choi R, Blackman MR, Arnold JT (2009) Endocrine-immune-paracrine interactions in prostate cells: A model for mechanistic studies of phytomedicines. Cancer Prev Res (Phila PA). 2(2): 134-42 (0798/lr/0003). 4. Fitzpatrick JL, Ripp SL, Smith NB, Pierce WM Jr, Prough R (2001) Metabolism of DHEA by cytochromes P450 in rat amd human liver microsomal fractions. Archives of Biochemistry and Biophysics. 389(2): 278-87 (0798/lr/0004). 5. Matsuzaki Y, Yoshida S, Honda A, Miyazaki T, Tanaka N, Takagiwa A, Fujimoto Y, Miyazaki H. Simultaneous determination of Dehydropiandrosterone and its 7-oxygenated metabolites in human serum by high- resolution gas chromatography
Additional info:	Recognizes dehydroepiandrosterone-sulphate.

See Full Product Listings >

DHEAS (Dehydroepiandrosterone Sulphate)

Cat no: D3228-03

Get Quote

About Biosave

Product Categories

Information


Get a Quote Direct from United States Biological




By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*