Data-independent acquisition (DIA) is a non-dependent data scanning mode, a holographic mass spectrometry data acquisition mode based on the electrostatic field orbitrap. After primary mass spectrometry detection, DIA fragments all parent ions within a specific m/z range, collects fragment ions of all parent ions, and quickly scans all fragment ions within adjacent wide windows of parent ions to perform protein qualitative and quantitative analysis. Compared to data-dependent acquisition mode (DDA), DIA has