DISC1 (Disrupted in Schizophrenia 1, RP4-730B13.1, KIAA0457, SCZD9)

DISC1 (Disrupted in Schizophrenia 1) is a 100-105kD cytoplasmic and mitochondrial protein that belongs to no known molecular family. It is widely expressed, and appears to have multiple interaction partners, among which are NDEL1, a-tubulin, TRAF3IP1 and GSK-3b. DISC1 is of particular interest in the brain where it appears to play a role in both neuronal proliferation and migration. Regarding proliferation, DISC1 inhibits GSK-3b activity, resulting in neural progenitor cell proliferation without differentiation. With respect to migration, DISC1 promotes embryonic subventricular neuron migration while inhibiting widespread adult neuronal migration from the hippocampal subgranular layer. Human DISC1 is 854aa in length. It contains an N-terminal globular domain (aa1-346) plus four coiled-coil regions (aa366-830). DISC1 is phosphorylated and forms homodimers. There are multiple isoforms. Among them is a 65-70kD form that shows an 18aa substitution for aa661-854, a 48kD form that contains a 20aa substitution for aa350-854, a 61-64kD form that possesses a seven aa substitution for aa545-854, and a 90kD form that contains a deletion of aa748-769. Over aa101-260, human DISC1 shares 44aa identity with mouse DISC1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.