DISP2, CT (Protein Dispatched Homolog 2, DISPB, KIAA1742)

Disp2 is the second of three known homologs of the D. melanogaster protein Dispatched. It is a multi-transmembrane protein containing two PTCH/DISP domains and is thought to be involved in the release of lipid-anchored Hedgehog from producing cells. Hedgehog is a major player in signaling pathways during embryogenesis, tissue regeneration, and carcinogenesis and the Disp proteins have been implicated in these pathways. Recently, it has been shown that Disp2 is translationally regulated by the microRNA miR-214 in zebrafish. Expression of this miRNA decreased Disp2 promoter activity in vitro and its overexpression in zebrafish resulted in a phenotype identical to that observed by Disp2 mutants. At least two isoforms of Disp2 are known to exist. This Disp2 antibody is predicted to not cross-react with Disp1 or Disp3.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nRat Brain Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146338

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Katoh Y and Katoh M. Identification and characterization of DISP3 gene in silico. Int. J. Oncol. 2005; 26:551-6. 2. Katoh Y and Katoh M. Hedgehog signaling pathway and gastric cancer. Can. Biol. & Ther. 2005; 4:1050-4. 3. Li N, Flynt AS, Kim HR, et al. Dispatched homolog 2 is targeted by miR-214 through a combination of three week microRNA recognition sites. Nuc. Acids Res. 2008; 36:4277-85.

Additional Info

Recognizes human Disp2. Species Crossreactivity: mouse and rat.