DJ-1, otherwise known as PARK7 (Parkinson disease protein 7), a member of the peptidase C56 family, highly expressed in skeletal muscle, heart, pancreas, liver, kidney and testis, which plays a role in transcriptional regulation and reaction to anti-oxidative stress.
DJ-1 exhibits cell-growth promoting and transforming activity, and protects neurons against oxidative stress and cell death. Defects in PARK7 are responsible for the autosomal recessive disorder early onset Parkinson disease 7.
Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions:
ELISA: 1:32,000
Western Blot: 0.5-1ug/ml detects a band of ~20kD in Jurkat cell lysates.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.