DJ-1 (PARK7, Protein DJ-1, Oncogene DJ1, Parkinson Disease Protein 7)

Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Mutations in a-synuclein, Parkin and DJ-1 have linked these genes to PD. Alpha-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice resulting in reduced long-term depression (6).\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3790-01H

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1.Borrelli, E. (2005) Neuron 45, 479-81. 2.Bonifati, V. et al. (2003) Science 299, 256-9. 3.Nagakubo, D. et al. (1997) Biochem. Biophys. Res. Commun. 231, 509-13. 4.Takahashi, K. et al. (2001) J. Biol. Chem. 276, 37556-63. 5.Mitsumoto, A. and Nakagawa, Y. (2001) Free Radic. Res. 35, 885-93. 6.Goldberg, M.S. et al. (2005) Neuron 45, 489-96.

Additional Info

Recognizes endogenous levels of total human DJ-1. It may also detect mouse and rat samples weakly.