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Dkk-2 (DKK2, SK, DKK-2, Dickkopf Homolog 2 (Xenopus laevis), Dickkopf-2 Like, Dickkopf (Xenopus laevis) Homolog 2)

Cat no: D3810-04

Dkk-2 (DKK2, SK, DKK-2, Dickkopf Homolog 2 (Xenopus laevis), Dickkopf-2 Like, Dickkopf (Xenopus laevis) Homolog 2)

Dickkopf (DKK) family proteins consist of four members DKK1, DKK2, DKK3 and DKK4 that function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5 and LRP6 (1,2). DKKs contain two cysteine-rich domains in which the positions of 10 cysteine residues are well conserved (3). Their expression is both temporally and spatially regulated during animal development (4). DKKs also bind with high affinity to transmembrane proteins Kremen1 and 2, which themselves also modulate Wnt signaling (5,6). DKK2 plays a role in osteoblast terminal differentiation and formation of mineralized matrix and is required for differentiation of non-keratinizing stratified epithelium from the corneal epithelial progenitors (7,8).\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3810-04

Size

100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

(1) Mao, B. et al. (2001) Nature 411, 321

Additional Info

Detects transfected levels of human DKK2 protein. This antibody does not cross-react with DKK1.

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