Dkk-2 (DKK2, SK, DKK-2, Dickkopf Homolog 2 (Xenopus laevis), Dickkopf-2 Like, Dickkopf (Xenopus laevis) Homolog 2)

Analysis of individual DKK domains and chimeric DKKs shows that the carboxy-terminal domains of both DKK1 and DKK2 associate with LRP6 and are necessary and sufficient for Wnt8 inhibition. Xenopus Dickkopf 1 (DKK1) was initially discovered as a Wnt antagonist that plays an important role in head formation. By far, four members of DKK have been identified in mammals. Each DKK molecule contains two conserved cysteine-rich domains. Recent studies showed that the second Cys-rich domains of DKK1 and DKK2 inhibited Wnt3a-activated signaling, whereas the first Cys-rich domains had no effects. In addition, the second Cys-rich domain of DKK2, but not that of DKK1, was able to activate the canonical pathway in the presence of LRP6, and this LRP-dependent signaling does not require Dvl.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3810-04A

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Xen/Amph

Form

Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Krupnik VE et al. Functional and structural diversity of the human Dickkopf gene family. Gene 238:301-13 (1999). PubMed: 10570958\nSearch PubMed (MEDLINE) for references to DKK2

Additional Info

Species Crossreactivity: Reacts with Human. Predicted to react with Mouse (100% identity with immunogen) and Xenopus laevis (86% identity with immunogen) due to sequence homology.