DLL1 (Drosophila Delta homolog 1)

DLL1(DELTA-LIKE 1) also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. Using FISH, Gray et al. (1999) mapped the DLL1 gene to chromosome 6q27, near a locus for type I diabetes. Functional analysis by Han et al. (2000) suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. Jaleco et al. (2001) used a cell coculture assay to show that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. In contrast, JAG1 did not disturb either B-cell or T-cell/NK-cell development. Galceran et al. found that mouse Lef1 bound multiple sites in the Dll1 promoter in vitro and in vivo, and mutation of the Lef1 sites impaired expression of a reporter transgene in the presomitic mesoderm of embryonic mice.\n\nUniProt Number:\nO00548\n\nGene ID:\nDLL1\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144451

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Gray, G. E., Mann, R. S., Mitsiadis, E., Henrique, D., Carcangiu, M.-L., Banks, A., Leiman, J., Ward, D., Ish-Horowitz, D., Artavanis-Tsakonas, S. Human ligands of the Notch receptor. Am. J. Path. 154: 785-794, 1999.\n2. Han, W., Ye, Q., Moore, M. A. S. A soluble form of human Delta-like-1 inhibits differentiation of hematopoietic progenitor cells. Blood 95: 1616-1625, 2000. \n3. Jaleco, A. C., Neves, H., Hooijberg, E., Gameiro, P., Clode, N., Haury, M., Henrique, D., Parreira, L. Differential effects of Notch ligands Delta-1 and Jagged-1 in human lymphoid differentiation. J. Exp. Med. 194: 991-1001, 2001.\n

Additional Info

Recognizes human, mouse and rat DLL1. No crossreactivity with other proteins.