DMBT1 (Deleted in malignant brain tumor-1; also MUCLIN, Hensin, CRP-Ductin and gp300/350) is a 230-350kD member of the DMBT1 family of molecules. It is expressed by select cell types including macrophages, intestinal epithelium, and pancreatic acinar cells. DMBT1 is both a transmembrane (TM) and secreted protein, and possesses multiple activities that may be predicated on multimerization. It drives epithelial differentiation, binds Gm+ and Gm- bacteria, and associates with cytoskeletal actin. Mouse DMBT1 is a type I TM glycoprotein that is 2085aa in length. Its extracellular region contains eight SRCR repeats (aa31-980, plus 1510-1610), five CUB domains (aa1023-1490, plus 1633-1742), and a ZP segment (aa1751-1999). Multiple splice variants exist. There are deletions of aa584-733, 397-534, 593-731 and 2032-2085 (within the TM segment), plus an insertion of 11aa after Asp29. Over aa215-420, mouse DMBT1 shares 75 identity with human DMBT1.
Applications:
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended Dilution:
Flow Cytometry:25ug/ml
ELISA: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Hybridoma:
Fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived,
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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