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DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1)

Cat no: D8074-90B

DMC (Dendritic cell and Monocyte Chemokine-like Protein, VEGF Co-regulated Chemokine 1, VCC1)

Dendritic cell and Monocyte Chemokine-like protein (DMC) is a secreted molecule that is closely related to CXCL8/IL-8 and CXCL14/BRAK. DMC is consitutively produced by airway and intestinal epithelium and induces the chemotaxis of monocytes and dendritic cells. It is strongly upregulated in proliferating vascular endothelial cells and some carcinomas. Mature human DMC shares 73% and 71% aa sequence identity with bovine and mouse DMC, respectively. \n\nApplications: \nSuitable for use in Flow Cytometry, Immunocytochemistry, ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 25ug/ml. 10ul labels 1-5x10e5 A549 cells in a total reaction volume (same/less than)200ul. For intracellular staining, cells must first be fixed and permeabilized using 4% paraformaldehyde, 0.1% saponin in PBS. \nImmunocytochemistry: 10ug/ml detects DMC in A549 cells. Cells were fixed with PBS, 4% paraformaldehyde and blocked with PBS, 10% normal donkey serum, 0.1% Triton X-100, 1% BSA. After blocking, cells were incubated with diluted D8074-90B followed by NL557-coupled anti-mouse IgG in the dark. Between each step, cells were washed with PBS, BSA.\nDirect ELISA: 0.5-1ug/ml. The detection limit is ~10ng/well.\nWestern Blot: 1-2ug/ml. Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D8074-90B

Size

100ug

Applications

ELISA, FC, ICC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b

Additional Info

Recognizes human DMC. Shows ~25% crossreactivity with recombinant mouse DMC. Species Sequence Homology: bovine, 73%; mouse, 71%

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