Mouse DMC (dendritic cell and monocyte chemokine-like protein; also VCC-1) is a secreted 11kD member of the CXC chemokine family. It is constitutively produced in lung, induced in endothelium by VEGF, and chemoattracts monocytes and dendritic cells. Mouse DMC is 97aa in length. It contains six cysteines with no potential N-linked glycosylation sites. Over aa31-119, mouse MDC shares 83% and 70% aa identity with rat and human MDC, respectively.
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution:
ELISA: 0.5-1.0ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.