DMPO Nitrone Adduct (Biotin)

The formation of free radicals and other highly reactive oxygen species has been implicated in the pathogenesis of many disease states. The ability to identify these species is crucial, and spin trapping has accomplished this goal. DMPO (5,5-dimethyl-1-pyrroline N-oxide) is one of the least toxic to cells and animals, and possesses convenient pharmacokinetics (uptake, distribution, metabolism and excretion) in biological systems. Recent studies have determined that nitric oxide may substantially affect the quantitative determination of DMPO adducts, and therefore extra caution is required when studying generation of these species in the presence of nitric oxide or its radicals. DMPO adducts can be generated with protein and DNA radicals.\n\nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1ug/ml detects the DMPO nitrone adducts of metmyoglobin when loaded at 100ng/lane by colorimetric blot analysis using IgG (HRP) goat anti-mouse as the secondary antibody\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D8075-94B

Size

100ug

Applications

ICC, IF, IHC, IP, WB

Hosts

Mouse

Conjugates

Biotin

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Reszka K.J., et al. (2006) Nitric Oxide 15: 133-141, 2. Ramirez D.C., Gomez-Mejiba S.E., and Mason R.P. (2007) Nat Protoc. 2(3): 512-522, 3. Khan N., et al. (2003) Free Radic. Biol. Med 34:1473

Additional Info

Recognizes DMPO Nitrone Adduct AT ~90kD. Also recognizes DMPO-octanoic acid, DMPO-protein adducts and DMPO-DNA adducts. Does not react with non-adducted proteins or DNA.

Alternative Names

5,5-dimethyl-2-(8-octanoic acid)-1-pyrroline N oxide