DNA

This clone is a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. It can also be used for sensitive detection of mycoplasma contamination in cell cultures. \n\nApplications:\nSuitable for use in Immunohistochemistry (frozen and paraffin-embedded tissue) and Dot blot on nitrocellulose membrane (after baking at 70 degrees C).\n\nRecommended Dilution:\nImmunohistochemistry: 1:10 with PBS, pH 7.4; Incubation Time 1hr at RT.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

D3875-01

Size

100ug

Applications

IHC

Hosts

Mouse

Reactivities

Xen/Amph

Form

Supplied as a lyophilized powder.

P Type

Mab

Purity

Gel filtration.

Isotype

IgM

References

Benavente, R. and Krohne, G.: Involvement of nuclear lamins in post mitotic reorganisation of chromatin as\ndemonstrated by microinjection of lamin antibodies. J. Cell Biol. 103, 1847-1854 (1986)\nHausman, P., Falk, H., Scheer, U. and Sitte, P.: Ultrastructural localization of DNA in two Cryptomonas species by\nuse of a monoclonal DNA antibody. Eur. J. Cell Biol. 42, 152-160 (1986)\nScheer, U.: Structure of lampbrush chromosome loops during different states of transcriptional activity as\nvisualized in the presence of physiological salt concentrations. Biol. Cell 59, 33-42 (1987)\nScheer, U., Messner, K., Hazan, R., Raska, I., Hausmann, P., Falk, H., Spiess, E. and Franke, W.W.: High\nsensitivity immunolocalization of double- and single-stranded DNA by a monoclonal antibody. Eur. J. Cell Biol. 43,\n358-371 (1987)

Additional Info

Reactivities on cultured XLKE-A6 (Xenopus), RVF-SMC, cell lines (tested so far) PTK-2, HeLa species (tested so far). Binding characteristics: Reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. Antigen recognized in All DNA-containing species.