DNA, ds

Applications: \nSuitable for use in EIA and Immunocytochemistry. Other applications not tested. \n\nRecommended Dilution:\nOptimal dilution determined by the researcher.\n\nBinding Assay: \nSupernatant was incubated with 32P-end labeled heat-denatured (ss)DNA or duplex (ds)DNA restriction fragments (20 pmoles nucleotides; 16,000 cpm) followed by precipitation of anti- DNA/DNA complexes with excess rabbit anti-mouse and quantitation of bound (32P) DNA by Chernkov radiation. \n\nAntigen Binding Profile:\nNatural DNA \ndsDNA(a) ssDNA(b)\n1.00 0.45\nSynthetic homopolymers \npoly(dA) poly(dC) poly(dG) poly(dT) poly(dI) poly(dU). \n0.01 0.01 0.01 0.01 0.01 0.01\n\nNucleic acids were complexed with solid phase mBSA and incubated with anti-DNA antibody. Radioiodinated protein A binding at the highest antibody quantity tested (either 10ug of purified antibody or a 1:2 culture supernatant dilution) was measured and normalized relative to that determined for the preferred antigen (given a value of 1.0). a) Native calf thymus DNA b) Heat-denatured calf thymus DNA \n\nStorage and Stability: \nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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