DNA, ds (Plasmid)

The dsDNA antigen is a 2690bp plasmid purified by alkaline lysis and chromatography. The purification process includes operations to minimize reactivity to antibodies against single-stranded DNA. Use of plasmid DNA in ELISA is an effective method for the detection of anti-double-stranded (ds) DNA antibodies (1). The presence of antibodies to dsDNA is included in the 1982 revised criteria for classification of Systemic Lupus Erythematosus (SLE) (2). Approximately 60-70% of SLE patients have antibodies to dsDNA, and there is considerable evidence to implicate immune complexes containing anti-dsDNA and DNA in the pathogenesis of SLE (3,4). In addition, levels of anti-dsDNA antibodies have been demonstrated to correlate with disease activity in many patients (5,6). Low levels of anti-dsDNA antibodies may occur in other rheumatic diseases (7) and may occur at a very low frequency (<2%) in individuals without any symptoms of rheumatic disease (8). Anti-dsDNA antibodies may appear in rheumatic patients prior to the development of disease manifestations (9).\n\nApplications:\nSuitable for use in standard solid phase immunoassays. Other applications not tested.\n\nPurity: Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. \n\nConcentration: ~1mg/ml\n\nForm: Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0.\n\nRecommended Dilutions: Optimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

D4015-06

Size

250ug

Applications

ELISA

Form

Supplied as a liquid in 0.01M Tris, 0.001M EDTA, pH 8.0.

Purity

Purified by isolating the Plasma DNA from E. coli by alkaline lysis and chromatographic methodologies. A260nm: 1.0 at 50ug/ml

References

1. Emlen W, et al. J.Immunol. Methods Vol 132:pg 91-101 (1990). 2. Tan EM, et al. Arthrits Rheum Vol 25:pg 1271-1277 (1982). 3. Nakamura RM, Peebles CL, Molden DP, Tan, EM. Lab. Medicine Vol 15: pg 190-198 (1984). 4. Condemi JJ. JAMA Vol 258: pg 2920-2929 (1987). 5. Tan EM, Schuer PH, Carr RI, Kunkel HG. J Clin Invest Vol 45: pg 1732-1740 (1966). 6. Miniter MF, Stollar BD, Agnello V. Arthritis Rheum Vol 22: pg 959-968 (1979). 7. Shoenfeld Y, Andre-Schwartz A, Stollar BD, Schwartz S. in Systemic Lupus Erythematosus, John Wiley & Sons, pg 213-255 (1987). 8. Stollar BD. Clin. Immun. Allergy Vol 1: pg 243-260 (1981). 9. Swaak AJG, et al. Annals of Rheum Dis Vol 41: pg 388-395 (1982).