DNA Ligase 3

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase 3 gene product migrates as a 100kD (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kD (beta-form) protein that is found in testis. DNA Ligase 3 is involved in rejoining DNA single-strand breaks and in the base excision repair pathway. \n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilutions:\nWestern Blot: 1-5ug/ml. Band at ~100kD.\nImmunohistochemistry (Paraffin): 1:10\nImmunoprecipitation: 1-5ug/ml \nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7, T24 cells and recombinant fusion protein. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

D3925-06

Size

100ug

Applications

IHC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. Preservative free.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

1. Lakshmipathy, U. and Campbell, C. (1999) The human DNA ligase III gene encodes nuclear and mitochondrial proteins. Mol Cell Biol 19:3869-3876. 2. lungland, A., et al. (1999) Base excision repair of oxidative DNA damage activated by XPG protein. Mol Cell 3:33-42. 3. Taylor, R.M., et al. (1998) Role of the DNA ligase III zinc finger in polynucleotide binding and ligation. Nucleic Acids Res 26:4804-4810. 4. Tomkinson, A.E. and Mackey, Z.B. (1998) Structure and function of mammalian DNA ligases. Mutat Res 407:1-9. 5. Cappelli, E., et al. (1997) Involvement of XRCC1 and DNA ligase III gene products in DNA base excision repair. J Biol Chem 272:23970-23975. 6. Nash, R.A., et al. (1997) XRCC1 protein interacts with one of two distinct forms of DNA ligase III. Biochemistry 36:5207-5211. 7. Wei, Y.F., et al. (1995) Molecular cloning and expression of human cDNAs encoding a novel DNA ligase IV and DNA ligase III, an enzyme active in DNA repair and recombination. Mol Cell Biol 15:3206-3216.

Additional Info

Recognizes human DNA Ligase 3.