DNA Marker 100bp DNA ladder is supplied at a total DNA concentration of 0.5mg/ml and is prepared from specially designed plasmid pME-80 DNA, containing pUC, lambda phage and yeast genome sequences.
The marker yields the following 10 discrete fragments (in base pairs): 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100. Convenient for analysis of PCR products up to 1 kb and can be used for DNA quantification.
The bright band at 500 bp serves as the reference.
Concentration:
0.5mg DNA/ml
Storage Buffer:
10mM Tris-HCl, pH 7.6, 1mM EDTA.
6x Loading Dye Solution:
Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.
Band Concentration:
0.5ug of DNA Ladder Mix contains:
a) 45ng (9.0%) for each of the 600-1000bp fragments.
b) 115ng (23.0%) for 500 bp band
c) 40ng (8.0%) for 100-400 bp bands.
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