The marker yields the following 15 discrete fragments (in base pairs): 20,000, 10,000, 7000, 5000, 4000, 3000, 2000, 1500, 1000, 700, 500, 400, 300, 200, and 75bp.
Band Concentration:
0.5ug of DNA Ladder Mix contains:
a) 25ng (5.0%) for 75- 400, 700 and 1000 bp bands
b) 75ng (15%) for 500 bp band
c) 80ng (16%) for 1500 bp band
d) 20ng (4.0%) for 2000-4000, 7000-20000 bp bands
e) 75ng (15%) for 5000 bp band.
6X Loading Dye Solution (Included):
Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60mM EDTA, 60% glycerol.
Quality Control:
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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