pBR322 DNA was completely digested by BsuRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The marker yields the following 22 discrete fragments (in base pairs): 587, 540, 502, 458, 434, 267, 234, 213, 192, 184, 124, 123, 104, 89, 80, 64, 57, 51, 21, 18, 11, 8.
Concentration: 0.5mg DNA/ml
Storage Buffer: 10mM Tris-HCl (pH 7.6), 1mM EDTA
6X Loading Dye Solution: 0.09% bromophenol blue, 0.09% xylene cyanol, 60% glycerol, 60mM EDTA.
Quantity of DNA in band:
Fragment (bp) % ng DNA
587 13.4 134
540 12.4 124
504 11.6 116
458 10.5 105
434 10.0 100
267 6.1 61
234 5.3 53
213 4.9 49
192 4.4 44
184 4.2 42
124 2.8 28
123 2.8 28
104 2.4 24
89 2.0 20
80 1.8 18
64 1.5 15
57 1.3 13
51 1.2 12
21 0.5 5
18 0.4 4
11 0.3 3
8 0.2 2
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