Plasmids were completely digested by the appropriate restriction endonucleases, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl (pH 7.6), 1mM EDTA. The digests were then mixed. The marker yields the following 21 discrete fragments (in base pairs): 10000, 8000, 6000, 5000, 4000, 3500, 3000, 2500, 2000, 1500, 1200, 1000, 900, 800, 700, 600, 500, 400, 300, 200, 100.
The bright bands at 3000 bp, 1000 bp and 500 bp serve as the reference.
Concentration:
0.5mg DNA/ml
Storage Buffer:
Supplied as a liquid in 10mM Tris-HCl, pH 7.6, 1mM EDTA
6x Loading Dye Solution:
(L3350)
Supplied as a liquid in 10mM Tris-HCl, pH7.6, 00.3% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.
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