DNA Marker Lambda EcoRI, 6 Fragments (3530-21226bp), BioGenomics(TM)

Lambda DNA was completely digested by EcoRI, phenol extracted, ethanol precipitated and dissolved in 10mM Tris-HCl, pH 7.6, 1mM EDTA. The marker yields the following 6 discrete fragments (in base pairs): 21226*, 7421, 5804, 5643, 4878, 3530*.\n\n*The cohesive ends of the 12 nt cos site of bacteriophage lambda from fragments of 21226 bp and 3530 bp may anneal and form an additional band at 24756 bp. These fragments may be separated by heating at 65 degrees C for 5 minutes and then cooling on ice for 3 minutes.\n\n6x Loading Dye Solution (L3350):\nSupplied as a liquid in 10mM Tris-HCl, pH 7.6, 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol, 60mM EDTA.\n\nQuantity of DNA in band:\nFragment % ng DNA\n(bp)\n21226 43.8 218.8\n7421 15.3 76.5\n5804 12.0 59.8\n5643 11.6 58.2\n4878 10.1 50.3\n3530 7.3 36.4\n\nTotal 100 500\n\nNote: \n1.One vial is sufficient for ~100 applications. \n2. Use 0.1ug of DNA marker (before dilution) per 1mm and an agarose gel lane.\n\nRecommended Use:\nVortex gently prior to use.\nPrepare DNA Marker as follows:\n 1ul (0.5ug) DNA marker\n 1ul of 6X Loading Dye Solution\n 4ul ddH2O\nHeat for 5 minutes at 65 degrees C. Cool on ice for 3 minutes. Apply the prepared amount (6ul) of the DNA Marker on a 5mm lane of agarose gel. Following electrophoresis separartion on gel, visualize the DNA bands by ethidium bromide staining.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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